Scaleview-a2 and 25x objective

Video 1

The image shows 8 mm deep acquisition of mouse brain transgenically encoded to express YFP in neurons.

Video 1

The image shows 4 mm deep acquisition of mouse brain transgenically encoded to express YFP in neurons.

Video 1

Image of YFP-H mouse hippocampus acquired as 24 region (4x6) montage over 3.3mm of depth, and stitched using Olympus FluoView software.

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  • Mag = 25x
  • N.A. = 0.9
  • W.D. = 8mm


Coming in February, the 8mm WD companion to the new SCALEVIEW 25x Objective Your Image.

SCALEVIEW-A2 Optical
Clearing Agent

Realize the impossible: 4mm deep imaging: Take Imaging to New Depths with Transparent-rendered Specimens and a Dedicated Objective

SCALEVIEW-A2 revolutionizes the imaging of formalin-fixed specimens. Simply render mammalian brain tissue transparent through immersion in SCALEVIEW-A2 solution. Because SCALEVIEW-A2 eliminates light scattering, it doesn't decrease the intensity of signals emitted by fluorescent proteins in the tissue and so enables structures labeled with fluorescent proteins to be imaged in detail from the surface to significant depth, without the need for mechanical sectioning.

SCALEVIEW-A2 contains the fundamental components of ScaleA2 developed by the RIKEN Brain Science Institute in Japan. It is adjusted to achieve the optimal performance with the XLPLN25xSVMP objective. (Read the RIKEN article in Nature Neuroscience at this link: http://www.nature.com/neuro/journal/vaop/ncurrent/full/nn.2928.html)

SCALEVIEW Immersion 25X Objective for Multiphoton

Specially designed to deliver optimum performance with SCALEVIEW immersion, this dedicated multiphoton objective with an ultra-long working distance enables high-precision imaging of transparent-rendered biological specimens to a depth of 4 mm.

Image data courtesy of:
Hiroshi Hama, Hiroshi Kurokawa, Atsushi Miyawaki
Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute
Reference material:
Hiroshi Hama, Hiroshi Kurokawa, Hiroyuki Kawano, Ryoko Ando,
Tomomi Shimogori, Hisayori Noda, Kiyoko Fukami,
Asako Sakaue-Sawano & Atsushi Miyawaki
"Scale: a chemical approach for fluorescence imaging and reconstruction of
transparent mouse brain," Nature Neuroscience, advance online publication, 30 August 2011 (doi:10.1038/nn.2928)

XLPLN25xSVMP specifications

Item Specifications
Numerical Aperture 1.0 (SCALEVIEW immersion)
Working Distance 4mm
Cover glass thickness(mm) 0 - 0.23mm
Field of view 18

SCALEVIEW-A2 specifications

Item Specifications
Optical characteristic Refractive index ne (23 °C) 1.380
Abbe number ve (23 °C) 51
Volume 1000 ml
Storage temperature 2 - 35 °C

Resources

Brochure
Scaleview Brochure

Articles:

  • Miyawaki, A., "Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain," Nature Neuroscience Read More
  • "New Way to Gain Clear View of the Brain," New York Times, October 10, 2011. Read More
  • "A new chemical reagent turns mouse brain transparent," RIKEN Brain Science Institute, press release, August 30, 2011. Read More

All images obtained using an Olympus FluoView FV1000-MPE multiphoton microscope system with either SCALEVIEW immersion 25x MPE 4mm or 8mm objective and SCALEVIEW-A2 optical clearing agent.

  • CX22IX3
  • Specialty Objectives DP80
  • CX22LV200
  • VS120 Slide SystemAndor
  • Imaris by Bitplane cellsens Digital Imaging Software