30X and 60X Silicone Oil Objectives
Fluorescence image of a transgenic zebrafish embryo at 26 hpf expressing zFucci. Courtesy of Dr. Mayu Sugiyama, Dr. Atsushi Miyawaki, Laboratory for Cell Function Dynamics, Advanced Technology Development Core, RIKEN Brain Science Institute
Image data courtesy of Hidehiko Inagaki, Anderson lab, California Institute of Technology. Image shows CFP and YFP labelling of glycerol cleared fruit fly brain taken with Olympus FluoView FV1000 confocal microscope using 30x silicone objective.
Minimizing spherical aberration and
delivering higher resolution at greater depths
- Improved optical performance for live cell confocal, wide-field fluorescence, multiphoton, and differential interference contrast (DIC)
- Ideal for long-term time-lapse imaging
- Enhance image resolution and contrast by adjusting correction collar
- 30X silicone objective for large field of view at high resolution
- High numerical apertures and transmission for use in both multiphoton and single-photon microscopy
Specifications
| Model | Magnification | N.A. | W.D.(mm) | Immersion Media |
Field of View |
Cover Glass Correction |
Temperature | DIC Observation |
|---|---|---|---|---|---|---|---|---|
| UPLSAPO60X | 60X | 1.3 | 0.3 | Silicone oil (ne=1.406) |
22 | 0.15-0.19mm | 23-37°C | Yes |
| UPLSAPO30X | 30X | 1.05 | 0.8 | Silicone oil (ne=1.406) |
22 | 0.13-0.19mm | 23-37°C | Yes |
Silicone Immersion Oil SIL300CS-30SC
- Refractive index: ne=1.406, 23C
- Net: 30ml
- Low autofluroescence
UPLSAPO30XS and UPLSAPO60X, Silicone
Immersion Objectives for Live Imaging
This immersion objective is designed exclusively for use with silicone oil, which has a refractive index even closer to live cells than that of water. Time-lapse observations become more reliable and less elaborate because silicone oil does not dry at 37°C and its refractive index remains constant. This objective also offers a long working distance to enable observation at deeper tissue levels and across broader fields. In a nutshell, this silicone objective offers a comprehensive solution for both large field of view and deep tissue observation in the fields of generative and regenerative science.
